Part:BBa_K933002:Experience

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Applications of BBa_K933002

This construct was used in our project to test different codon repeat sequences. There are two restriction sites: BamHI and HindIII flanking the repeat sequence site. The repeat sequences were designed to be ligated into the construct at that site. By placing in repeat sequences of codons, the codon bias was tested based on the RFP expression. The second RBS30 site was placed there so that GFP would be expressed constant.

This construct was used for testing of the Threonine codon 6 repeats:

Graph of results:

User Reviews

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